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2013: Product keys for Autodesk products.. Autodesk 3ds Max 2013: 128E1: Autodesk 3ds Max Design 2013:. Find Serial Number & Product .Some bacteria use a crystal membrane as a storage depot for fatty acids and use glycogen-like storage granules to temporarily store energy reserves. We are investigating a different form of energy reserve in Myxococcus xanthus, a non-proliferating bacterium that forms multicellular fruiting bodies. We recently found that M. xanthus has an osmosensing channel (OsP1). In M. xanthus, the OsP1 homologue, OsP2, is secreted by an ESAT-6-like protein secretion system and accumulates on the cell surface at high osmolarity. In S. cerevisiae, OSH1, an osmosensing channel, is encoded by a member of the major facilitator superfamily (MFS). It is unclear if M. xanthus OsP2 has a function similar to OSH1. We found that OsP2 is not an MFS protein, and that this channel has a unique mechanism for regulation. We hypothesize that OsP2 is an osmosensing protein that plays a role in the accumulation of M. xanthus energy reserves. In the first aim, we will define the molecular characteristics of OsP2 by identifying the type of amino acids that make up OsP2, and by cloning and sequencing OsP2 cDNA. In aim 2, we will characterize the channel properties of OsP2 using electrophysiological techniques, and determine if there is a relationship between the OsP2 potassium channel and the function of energy reserves. In aim 3, we will characterize the role of OsP2 in the accumulation of energy reserves by expressing OsP2 in osP2 mutant cells and determining the accumulation of energy reserves in cells with impaired OsP2. This study will contribute to our understanding of osmosensing by 0b46394aab